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The DPPH radical scavenging activity is generally quantified in terms of inhibition percentage of the pre-formed free radical by antioxidants, and the EC 50 (concentration required to obtain a 50% antioxidant effect) is a typically employed parameter to express the antioxidant capacity and to compare the activity of different compounds. DPPH assay is a reliable method to determine the antioxidant capacity of biological substrates. Its solution helps you pinpoint areas that need improvement so your business can take a step ahead of the competition.IBM SPSS offers adequate ROI to your company even in the aspect of hiring. Using predictive analysis helps you find the right talents in an instant. Through this, you can retain skilled and valuable employees while reducing the losses that happen because of elevated levels of attrition. With IBM SPSS, you can use predictive models to adapt it to your business process and your customers’ needs, giving you both an insightful and intimate relationship.
All the statistical programs gave similar EC 50 values, but GraphPad Prism® five-parameter analysis pointed out a best performance, also showing a minor variance in relation to the actual EC 50. For this project, quercetin, catechin, ascorbic acid, caffeic acid, chlorogenic acid and acetylcysteine were screened as antioxidant standards with DPPH assay to define the EC 50 parameters.
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